Exons 2 to 6 were flanked by loxP sites. A FRT-flanked PGK-neo cassette was inserted upstream of the floxed exons. Flp-mediated recombination removed the selection cassette, leaving exons 2-6 floxed. (J:283622)
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Exons 2 to 6 were flanked by loxP sites. A FRT-flanked PGK-neo cassette was inserted upstream of the floxed exons. Flp-mediated recombination removed the selection cassette, leaving exons 2-6 floxed. (J:283622)