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The targeting vector contains a 5.5 kb 5 arm and a 3.5 kb 3arm. Lox71 and Lox2272 sites were inserted flanking the gene, where the cDNA was replaced with that encoding for an HA-tagged Ngn3 protein. The vector also contains a FRT-flanked Pgk-Puro-deltaTK cassette for positive selection by puromycin (targeting events) and negative selection by ganciclovir (RMCE events). (J:296396)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
