Recombinase-mediated cassette exchange replaced the coding sequence with a cre/ERT2 fusing using the loxed cassette allele (LCA). Flp-mediated recombination removed the FRT-flanked hygromycin cassette. (J:296396)
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Recombinase-mediated cassette exchange replaced the coding sequence with a cre/ERT2 fusing using the loxed cassette allele (LCA). Flp-mediated recombination removed the FRT-flanked hygromycin cassette. (J:296396)