Dpp3^{tm1d(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 4970581 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4971737. The critical exon(s) is/are thus flanked by loxP sites (creating the Dpp3tm1c(EUCOMM)Hmgu allele). The floxed targeted exons were deleted through cre-mediated recombination in mice carrying the tm1c allele, creating this tm1d knockout allele. Semi-quantitative RT-PCR on several tissues confirmed lack of transcript expression and Western blot analysis confirmed the absence of protein expression in total bone. (J:294242)