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A crRNA (5-CAACCAGTTGTTTGGCCAAC-3) targeting exon 1 and a trans-activating crRNA (tra-crRNA) were used with CRISPR/Cas9 technology to create a knockout allele. An ssODN (5-TCTTCATCACTGGCTGTGACTCCGGCTTTGGGAATCTGTTGGCCAAACAACTGGTTGATAGGGGCATGAAAGTGCTTGCT-3) was included to include a silent (synonymous) c.117C>T mutation to avoid cleavage by Cas9, reducing the chances of creating potentially embryonic lethal bi-allelic mutations. The resulting mutation is a single basepair deletion (c.123delG) in exon 1. Lack of protein expression from this allele was confirmed by immunohistochemistry. (J:295441)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
