Kbtbd13^em1Coeo

Two sgRNAs and an ssODN template were used with CRISPR/Cas9 technology to create a C-to-T mutation (G-to-A on forward strand) that changes codon 408 from arginine to cysteine (p.R408C). This mutation is equivalent to the one found in nemaline myopathy (NEM6) patients. (J:295442)