The targeting vector is designed to insert a loxP site into the 5' UTR, upstream of the first coding exon (exon 2). A second loxP site is inserted downstream of the 3' UTR, followed by a cassette containing an FRT-flanked PGK-neo and a third loxP site. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 2 floxed. (J:287983)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count