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CRISPR/Cas9 genome engineering was used to generate mice with a knockin mutation that alters codon 271 from one encoding aspartic acid to one encoding alanine. This residue is part of the YD and GXGD active site motif that is highly conserved among intramembrane aspartyl proteases and whose mutation to alanine has been shown to abrogate protease activity. This allele is described as "protease-dead". (J:280182)