A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop.The LacZ-neo cassette was removed through flp-mediated recombination and the stem loop was removed through subsequent cre-mediated recombination. (J:294951)
Basic Information
Insertion, Intragenic deletion
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count