Coq8b^{tm1c(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 26938245 of Chromosome 7 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 26939943. Exons 5 and 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:294296)