Tg(CAG-mRuby2*,-mClover*)1Srgt

CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CMV enhancer/chicken beta-actin (CAG) promoter followed by a loxP-flanked STOP cassette, an H2B/mRuby2 fusion protein, a P2A self-cleaving peptide sequence, and an ERK-KTR/mClover fusion protein into the Gt(ROSA)26Sor locus. ERK-KTR consists of a docking site (a fragment of human ELK1, an ERK substrate), nuclear localization (NLS) and export (NES) signals, and mClover green/yellow fluorescent protein. Through nanopore sequencing and PCR verification, it was determined that the vector, along with the Rosa26 homology arms, randomly inserted into a noncoding region of chromosome 13. (J:297084)