The targeting construct was designed to insert a loxP site upstream of exon 2 and an FRT-flanked neomycin cassette followed by a second loxP site downstream of exon 3. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:293926)
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The targeting construct was designed to insert a loxP site upstream of exon 2 and an FRT-flanked neomycin cassette followed by a second loxP site downstream of exon 3. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:293926)