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RMCE (recombinase-mediated cassette exchange) was utilized to insert a Myc-tagged human CEBPB sequence into the Gt(ROSA)26Sor locus. R26FNF3-1F1 (1F1) embryonic stem (ES) cells were transfected with plasmids pFLSLF2-Myc human CEPBP (carrying loxP pA-puromycin-PGK-Sv40 pA (in reverse orientation)-loxP-Myc-CEBPB-WPRE-BGH pA) and pCAG-Flpe (expressing FLP recombinase). The 1F1 ES cell line used to generate the mutation was created by targeting Gt(ROSA)26Sor with an FRT- and F3-flanked PGK-neomycin selection cassette by homologous recombination in (C57BL/6J x 129X1/SvJ)F1-derived EC7.1 ES cells. (J:286809)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
