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EN
Double recombinase mediated cassette exchange (dRMCE) was used to replace exon 1 of the endogenous gene with a 3xFLAG epitope tagged exon1. An unmodified exon 2 replaced endogenous exon 2 and an attB/attP flanked PGK-hygro selection cassette was inserted at the same time. Modification was carried out in Hand2 ES cells in which exons 1 and 2 were flanked by loxP sites and an FRT flanked neomycin cassette was inserted downstream. Replacement was accomplished by simultaneous cre and flp recombinase expression in the ES cells. (J:263215)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
