The targeting vector is designed to insert a loxP site upstream of exon 2, and a second loxP site followed by an FRT-flanked PGK-neo cassette just downstream of exon 3. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:292023)
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The targeting vector is designed to insert a loxP site upstream of exon 2, and a second loxP site followed by an FRT-flanked PGK-neo cassette just downstream of exon 3. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:292023)