The sequence just upstream of the stop codon was targeted for insertion of HA tag sequence with a gRNA (5'-GGGACCTACAGCAGAATAG-3') and an oligonucleotide donor, using CRISPR/Cas9 technology. The resulting allele has sequence coding for nine C-terminal haemagglutinin (HA) amino-acids inserted in-frame immediately upstream of the stop codon. (J:291761)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
Insertion
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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