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CRISPR/Cas9 genome editing is used to insert a T2A-Cre cassette immediately upstream of the endogenous stop codon, a 1kb upstream homology arm, and a 2kb downstream homology arm with a TG to GT mutation introduced into the 3' UTR (114-115bp after stop codon) to avoid cleavage by the sgRNA. The T2A oligopeptide mediates ribosomal skipping and is derived from foot-and-mouth disease virus 2A from the insect Thosea asigna virus. T2A permits cre recombinase to be expressed in frame with Uts2b as a separate polypeptide. (J:291579)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
