The following were introduced to intron 3: FRT-En2 SA-IRES-lacZ-pA-loxP-hbactP-Neo-pA-FRT-loxP, and a loxP site was placed in intron 4. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassettes. (J:157065)
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The following were introduced to intron 3: FRT-En2 SA-IRES-lacZ-pA-loxP-hbactP-Neo-pA-FRT-loxP, and a loxP site was placed in intron 4. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassettes. (J:157065)