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EN
CRISPR/Cas9 genome editing is used to replace the stop codon with a bicistronic reporter containing a P2A peptide sequence and the fluorescent reporter ametrine. In addition, an in-frame 9 nucleotide deletion is found near the 3' end of the gene. The deletion is not believed to affect protein expression. Ametrine is a long stokes shift (excitation at 406 nanometers and emission at 526 nanometers) yellow fluorescent protein derived from Aequorea victoria. (J:259930)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
