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CRISPR/Cas9 endonuclease-mediated genome editing was used to insert a T4709M missense mutation in exon 96 of the gene. The mutation is predicted to result in an amino acid substitution at position 4709 altering threonine to methionine (c.14117 C to T). The mutation is orthologous to the human variant T4706M. Sequencing of N1 mice revealed only the c.14117 CtoT, p.T4706M was incorporated, and not the silent c.14100 C to T change. (J:275500)