Dtl^{tm1c(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 191293225 of Chromosome 1 upstream of the critical exons 6-7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 191295597. The critical exons 6-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele, to remove the lacZ sequence and selection cassette, leaving loxP sites flanking the critical exons 6-7. (J:245637)