The targeting construct inserted into the locus an F3 site, neomycin resistance (NeoR) cassette containing a polyadenylation signal and lacking a translation initiation codon, CAG promoter cassette (containing a cytomegalovirus early enhancer element, a chicken beta-actin promoter and an adenovirus intron), the Acad10 open reading frame (based on NCBI transcript NM_028037_2), a synthetic polyadenylation signal, and FRT site. (J:147112)