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The strand guide RNA (sgRNA) was designed to bind to ~20 nucleotides within the Ppif (CypD) locus and was centered near the nucleotides that predict p.Cys202Ser (C202S). Oligo2 containing four mutations (see section Genetic Alteration) was injected with the CRISPR/Cas9 components to be incorporated into the genomic DNA via homologous recombination at the cut CypD locus. Recombination of Oligo2 produced a CypD locus with the aforementioned 4 exchanges, three silent and the fourth predicting a cysteine-to-serine substitution at codon 202. (J:94077)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
