LoxP sites were inserted to flank exons 4-7. An frt-flanked neomycin selection cassette was inserted 5' to the second loxP site in intron 7. Cre-mediated recombination removed exons 4-7 and the selection cassette. (J:261055)
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LoxP sites were inserted to flank exons 4-7. An frt-flanked neomycin selection cassette was inserted 5' to the second loxP site in intron 7. Cre-mediated recombination removed exons 4-7 and the selection cassette. (J:261055)