Setbp1^em8Lutzy

Guide RNAs [TCCCGATGCCACTGTCGCTC and GAGACTATCCCGAGCGACAG] were originally designed to introduce a point mutation in exon 4. DNA sequencing of the targeted region identified genome edited pups harboring a Setbp1 98-nt frameshift deletion (indel mutation) that starts at Pro-857 and introduces a TGA stop codon immediately afterwards [GCGAGGAGACTATCCC//del98//TTGACAACCCAGAGGCC]. (J:101977)