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The targeting vector is designed to insert a HaloTag cDNA sequence (see below), a TEV protease recognition sequence and an in-frame loxP-STOP-IRES-FLAG-loxP cassette upstream of the first coding exon (exon 1), and an FRT-flanked PGK-neo cassette in intron 1 of the gene. HaloTag is a 33 kDa haloalkane dehalogenase encoded by the DhaA gene from Rhodococcus rhodochrous that has been mutagenized to form an irreversible covalent bond to synthetic chloroalkane ligands. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:283893)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
