A loxP site was inserted upstream of the coding sequence. A Venus gene with a 5' loxP site and an FRT-flanked neo cassette were inserted downstream of the 3' UTR. Flp-mediated recombination removed the selection cassette. (J:174732, J:217912)
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A loxP site was inserted upstream of the coding sequence. A Venus gene with a 5' loxP site and an FRT-flanked neo cassette were inserted downstream of the 3' UTR. Flp-mediated recombination removed the selection cassette. (J:174732, J:217912)