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The 176 kb bacterial artificial chromosome, RP24-256F2, containing the entire Chat and Slc18a3 (VAChT) genes was modified to insert an optimized FLP recombinase (FLPo) followed by a roxP-flanked PGK-prokaryotic gb2 promoter-neoR/kanR cassette under the direction of the Chat promoter. Induction of Dre recombinase removes the kanamycin cassette in recombineering competent bacteria. Next, a 3X Stop is inserted into the Slc18a3 (or VAChT) blocking transcription. (J:101977)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
