Nr3c1^tm2.1Jaci

A targeting exchange vector was designed to contain exon 2 in which the ATG start codons for the translational isoforms GR-A, GR-B, GR-C1, GR-C2, GR-D1, GR-D2, and GR-D3 were mutated to ATC (isoleucine) except the start codon for GR-C3, which was left intact. In addition, a lox66 site followed by a hygromycin resistance gene cassette flanked by FRT sites was placed into intron 1 and a lox2272 site downstream of the mutated exon 2. This exchange vector and cre recombinase were transfected into embryonic stem cells in which exon 2 was replaced by a neomycin resistance gene cassette flanked by an upstream lox71 site and downstream lox2272 site. Cre-mediated recombination results in exchange of the neo cassette with the mutated exon 2. Flp-mediated recombination removed the hygro cassette. Mice exclusively express the GR-C3 isoform, which lacks the first 97 aa of the N-terminal domain. (J:282764)