A loxP-FRT-PGK-Neo-PGKpA-FRT cassette was introduced to intron 4 and a loxP site was placed in intron 5. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 5 floxed. (J:282819)
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A loxP-FRT-PGK-Neo-PGKpA-FRT cassette was introduced to intron 4 and a loxP site was placed in intron 5. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 5 floxed. (J:282819)