Tnni3k^em1Hsv

CRISPER/Cas9 genome editing is used to change the lysine codon at position 489 to arginine (AAA to AGA) in exon 15 of the gene. This mutation results in a premature stop codon homologous to one found in exon 16, early in the encoding portion of the kinase domain, in the naked mole-rat species Heterocephalus glaber. Two G-to-T substitutions were also introduced in order to eliminate the Cas9 PAM sequence to prevent retargeting. (J:280429)