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CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CAG-H2B-miRFP703 (nuclear localized) vector into the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette. The STOP cassette was removed by an in vitro cre reaction on the plasmid. miRFP703 is derived from miRFP670, which is itself derived from RpBphP1, a bacterial phytochrome photoreceptor, Rhodopseudomonas palustris. miRFP703 includes the following mutations: D16S/L17H/C20S/R22H/P43H/I47V/D106C/P127S/A155V/E179L/R184C/D201T/I202V/R208Q/E211V/S222T/I253C/Y257F/N260D/V268A/A282V/H292R/E300K/L301R/A305R/S20C/T201L/C253I. (J:266345)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
