The targeting vector contains a loxP site placed in intron 5 and an FRT::neomycin-delta thymidine kinase (Neo-DTK)::FRT::loxP cassette placed in intron 7. The Neo-DTK cassette was removed in vitro by transfecting with Flp recombinase. (J:282446)
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The targeting vector contains a loxP site placed in intron 5 and an FRT::neomycin-delta thymidine kinase (Neo-DTK)::FRT::loxP cassette placed in intron 7. The Neo-DTK cassette was removed in vitro by transfecting with Flp recombinase. (J:282446)