Fgf18^{tm2.2(cre/ERT2)Dor}

A multifunctional targeting vector inserted a splice acceptor (SA)-eGFP:CreERT2 fusion gene into the intron located between Fgf18 exons 1B and 1C along with a PGK-neo selection cassette. Additional Frt and LoxP recombination sites were placed in the targeted allele to allow the generation of two independent alleles by crossing the parental mouse line to either a germline expressed FLP recombinase or a germline expressed Cre recombinase mouse. This generated a tamoxifen inducible eGFP:CreERT2 allele driven by the endogenous Fgf18 promoter (Fgf18CreERT2) that can be used for lineage tracing. The Fgf18CreERT2 allele has exon 1C deleted and is predicted to behave as a functional null allele. (J:279323)