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First, a single guide RNA (5'-TGAACATTCTGACAAGACCC-3') was designed to target sequences in exon 2. Next, a DNA oligo for homology directed repair (HDR) was designed to target a 33 bp insertion (BamHI site, Flag tag sequence and STOP sequence) into exon 2 that introduced a premature stop codon at amino acid 93 (P83delinsGSDYKDDDDK*). (J:281519)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
