A loxP site and a frt-flanked neomycin resistance gene were inserted upstream of exon 4 and a second loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette leaving exon 4 floxed. (J:265659)
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A loxP site and a frt-flanked neomycin resistance gene were inserted upstream of exon 4 and a second loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette leaving exon 4 floxed. (J:265659)