A loxP site was inserted upstream of exon 1 and an FRT flanked pgk-neo cassette and loxP site were inserted downstream of exon 1 via homologous recombination. Flp mediated recombination removed the pgk-neo cassette. (J:279065, J:280829)
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A loxP site was inserted upstream of exon 1 and an FRT flanked pgk-neo cassette and loxP site were inserted downstream of exon 1 via homologous recombination. Flp mediated recombination removed the pgk-neo cassette. (J:279065, J:280829)