Bag3^tm2.1Chen

A C to T mutation (c.644C>T) was engineered in exon 3 to change proline codon 215 to a leucine codon (p.P215L). This mutation mimics the p.P209L mutation associated with severe childhood cardiomyopathy in humans. An FRT site flanked neomycin resistance gene cassette that was inserted into intron 2, was removed through subsequent flp-mediated recombination. (J:272259)