A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. The FRT-flanked neo cassette was removed via Flp-mediated recombination in the germline. (J:269219)
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A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. The FRT-flanked neo cassette was removed via Flp-mediated recombination in the germline. (J:269219)