Afg3l2^tm1.1Alfb

Using a recombineering approach, a c.1994T>G point mutation was engineered in exon 16 to change codon 665 from methionine to arginine (p.Met665Arg). The FRT site flanked neomycin resistance gene cassette that was inserted into intron 15, was removed through subsequent flp-mediated recombination. This mutation mimics the p.Met666Arg mutation found in human spinocerebellar ataxia 28 (SCA28) patients. (J:277510)