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RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Rpl5 into the Col1a1 locus. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a turboGFP sequence (tGFP; a fast-maturating and short-half-life green fluorescent protein variant) and short-hairpin RNA sequence targeting Rpl5 embedded in the MIR30 structure. The vector was then co-electroporated with a FLPe recombinase-expressing vector into the RMCE-ready KH2 ES cells. (J:341566)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
