Using CRISPR/Cas9 technology with sgRNA 5'-GCCTCGGCGGGCATCCCGCA-3', sequence for the P2A self-cleaving peptide sequence plus the tdTomato red fluorescent reporter gene was inserted immediately before the stop codon. (J:293995)
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Using CRISPR/Cas9 technology with sgRNA 5'-GCCTCGGCGGGCATCCCGCA-3', sequence for the P2A self-cleaving peptide sequence plus the tdTomato red fluorescent reporter gene was inserted immediately before the stop codon. (J:293995)