Pigu^{tm1c(EUCOMM)Hmgu}

The L1L2_st1 cassette was inserted at a position on Chromosome 2 upstream of exon 2 of the gene. The cassette is composed of an FRT-flanked lacZ sequence fused to a neomycin sequence followed by a loxP site. A second loxP site was inserted downstream of exon 3. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 3. (J:303166)