Dspp^em1Yolu

Using CRISPR/Cas9 technology and an sgRNA and an ssODN donor, exon 2 was targeted with a c.56C>T mutation to change codon 19 from proline to leucine (p.P19L). Additionally, a silent c.45G>C mutation was introduced into the third base of alanine codon 15. The P19L substitution is the equivalent of the P17L mutation found in human dentinogenesis imperfecta (DGI) type II and type III patients. (J:278819)