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The ~228 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-7M9 was obtained; containing the protein coding region of the Aldh1l1 as well as ~170 kbp of telomeric flanking sequences (including the complete Slc41a3 locus and a portion of the Iqsec1 locus). Using homologous recombination/BAC recombineering, a 6170 bp Opto alpha1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Aldh1l1 gene. No other loci on the BAC were altered. The 11.6 kbp BAC vector contained a chloramphenicol selection cassette. The fusion protein is a Gt-coupled bovine rhodopsin sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gq-coupled human ADRA1A. (J:314125)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
