Wt1^{em1(cre/ERT2)Bzsh}

The mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. A complementary DNA encoding Cre recombinase fused with 2 rox sequences flanking a mutant form of the ESR hormone-binding domain (ERT2) was inserted into the translational start site of the Wt1 gene and followed by a polyadenylation sequence. (J:278094)