A loxP site and FRT flanked neo cassette were inserted in intron 1 and a loxP site was inserted in intron 4 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2 - 4. (J:271630)
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A loxP site and FRT flanked neo cassette were inserted in intron 1 and a loxP site was inserted in intron 4 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2 - 4. (J:271630)