Sequence coding for an N-terminal hemagglutinin (HA) tag was inserted into exon 1 immediately after the ATG start codon, isoleucine codon 73 (ATC) in exon 3 was changed to threonine (p.I73T), and an FRT site flanked neomycin resistance gene and gb2-promoter-driven kanamycin resistance gene cassette into intron 1. The neo/kana cassette was removed through subsequent Flp-mediated recombination. (J:267027)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion, Nucleotide substitutions
--
1
5
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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