A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette plus a second loxP site were inserted into intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. (J:238746)
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A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette plus a second loxP site were inserted into intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. (J:238746)