The region encompassing exons 4 and 5 was flanked by loxP sites. A loxP site was inserted into the intron between exons 5 and 6 by PCR mutagenesis and a floxed neomycin resistance (neo) cassette was inserted between exons 3 and 4. (J:122087)
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The region encompassing exons 4 and 5 was flanked by loxP sites. A loxP site was inserted into the intron between exons 5 and 6 by PCR mutagenesis and a floxed neomycin resistance (neo) cassette was inserted between exons 3 and 4. (J:122087)